Department of Animal Breeding

Determination of exogenous antioxidants in milk, colostrum and meat: tocopherol (vitamin E), carotenoids (b – carotene), retinol (vitamin A), vitamin C
Determination of endogenous antioxidants in milk, colostrum and meat: functional fatty acids, lactoferrin, albumin, glutathione, super oxide dismutase (SOD – Super Oxid Dismutase)
Determination of the fatty acid content in milk, colostrum and meat: saturated fatty acids SFA: C4: 0, C6: 0, C8: 0, C10: 0, C12: 0, C14: 0, C15: 0, C16: 0, C17: 0, C18: 0, C20: 0, C21: 0, C23: 0, C22: 0, C24: 0; monounsaturated fatty acids MUFA: C10: 1, C12: 1, C14: 1, C15: 1, C16: 1, C17: 1, TVA, C18: 1, C20: 1, C22: 1, C24: 1; PUFA polyunsaturated fatty acids: LA, CLA, GLA, DGLA, LNA, ARA, EPA, DPA, DHA; Conjugated CLA linoleic acid isomers, branched fatty acids (BFA)
Determination of the content of whey proteins in milk and colostrum: lysozyme, lactoferrin, α-lactalbumin, β-lactoglobulin, lactoperoxidase, immunoglobulins IgM, IgA and IgG
Determination of the content of dipeptides in milk, colostrum, meat: lactoferrin, carnosine, anserine
Determination of the content of fat-soluble vitamins and β-carotene in milk and colostrum: tocopherol, carotenoids, retinol, menaquinone, cholcecalciferol
Determination of cholesterol and phospholipids content: cholesterol, phospholipids (phosphatidylcholine, sphingomyelins, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine

Agilent 1100 liquid chromatograph equipped with a 4-valve gradient pump, autosampler for 100 samples, UV-Vis detector and specialized capillary columns, fraction collector
Agilent 7890A gas chromatograph with an automatic feeder for 150 samples, equipped with a FID detector and specialized capillary columns
Analytical methods: chromatographic separation techniques, derivatization of fatty acids into methyl esters (FAME) is carried out in accordance with the EN ISO 5509 procedure, fat-soluble vitamins and β-carotene are determined using reversed phase HPLC (RP-HPLC) and Agilent apparatus 1100 equipped with a ZORBAX Eclipse XDB – C8 column, 4.5 mm x 150 mm in diameter, whey proteins: Lz, Lf, a-LA, BSA, b-LG are determined using RP-HPLC and Supelcosil LC-318 column